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TRIMGALORE OOM-killed (137) on all samples in 3.26.0; works in 3.23.0

bugSomething isn't workingSomething isn't working

Status: Open.

#1903 In nf-core/rnaseq;

· JosephLongworth opened on Aug 13, 2026

Error code 127

Status: Open.

#1898 In nf-core/rnaseq;

· lpgarkins opened on Jul 28, 2026

STAR_ALIGN fails with Transcriptome.cpp:18: could not open input file /geneInfo.tab

bugSomething isn't workingSomething isn't working

Status: Open.

#1897 In nf-core/rnaseq;

· ftucos opened on Jul 28, 2026

Add support for non-Ensembl annotation files

enhancement

Status: Open.

#1886 In nf-core/rnaseq;

· murphyte opened on Jul 16, 2026

--use_rustqc + --with_umi still fails at RUSTQC — the #1869 validation does not cover the UMI path

Status: Open.

#1881 In nf-core/rnaseq;

· FelixKrueger opened on Jul 6, 2026

Reorganise usage docs with a user-centric structure

documentation

Status: Open.

#1737 In nf-core/rnaseq;

· pinin4fjords opened on Feb 27, 2026

Refactor empty meta map pattern in main.nf

Status: Open.

#1723 In nf-core/rnaseq;

· pinin4fjords opened on Feb 26, 2026

More than 1 reference genome

enhancement

Status: Open.

#1695 In nf-core/rnaseq;

· gfill88 opened on Feb 9, 2026

Investigate --alignMatesGapMax for STAR prokaryotic alignment

Status: Open.

#1691 In nf-core/rnaseq;

· pinin4fjords opened on Feb 5, 2026

Sylph Performance Improvement

enhancement

Status: Open.

#1670 In nf-core/rnaseq;

· egreenberg7 opened on Dec 19, 2025

Update StringTie to latest version

enhancement

Status: Open.

#1506 In nf-core/rnaseq;

· rrdavis77 opened on Feb 14, 2025

3.16.x does not allow numeric sample ids

bugSomething isn't workingSomething isn't working

Status: Open.

#1419 In nf-core/rnaseq;

· idot opened on Oct 17, 2024